rabbit anti mouse rad51 santa cruz biotecnology h92# sc-8349  (Santa Cruz Biotechnology)

Journal: Stem Cell Reports

Article Title: Endogenous DNA Damage Leads to p53-Independent Deficits in Replicative Fitness in Fetal Murine Fancd2 − / − Hematopoietic Stem and Progenitor Cells

doi: 10.1016/j.stemcr.2016.09.005

Figure Lengend Snippet: DNA-Damage Responses Are Induced, and the Strand Breaks Are Accumulated in FA FL HSPCs (A) Representative γH2AX foci in SCA1 + FL cells (60× objective lens; the scale bar represents 5 μm). (B) Quantification of γH2AX foci per cell (n = 3 WT and 3 KO from three litters). (C) Representative RAD51 foci in SCA1 + FL (60× objective lens; the scale bar represents 5 μm). (D) Quantification of cells positive for foci (p = 0.02, n = 6 WT and 6 KO from three litters). (E) Expression of DDR genes in SCA1 + FL cells (p = 0.08, 0.44, 0.06, 0.22, and 0.005, respectively; n = 4 WT and 4 KO from three litters). (F) Expression of selected DDR genes in ASL-sorted FL cells (p = 0.08, 0.5, 0.04 respectively; n = 3 WT and 3 KO from two litters). (G) Olive tail moment of 428 SCA1 + FL cells from seven WT animals and 288 SCA1 + FL cells from five Fancc − / − animals from four litters (p = 0.003). (H) Olive tail moment of 289 SCA1 + FL cells from five WT animals and 267 SCA1 + FL cells from five Fancd2 − / − animals from four litters (p = 0.04). (I) Representative alkaline comets of SCA1 + FL cells (20× objective lens; the scale bar represents 20 μm). Error bars reflect SEM, and asterisks indicate ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01.

Article Snippet: Cells were permeabilized with NET buffer with 0.5% Triton X-100 ( ) and stained with Hoechst (Thermo Scientific 62249) and AF488-conjugated anti-γH2AX (BioLegend, 613407), rabbit anti-mouse RAD51 (Santa Cruz Biotechnology, 8349) followed by Cy3 goat anti-rabbit IgG (Millipore, AP132C) or Cy5-conjugated anti-phospho-p38 (T180/Y182) (eBioscience, 17-9078-42).

Techniques: Expressing